Diagnosis of human cytomegalovirus with renal failure by RT-qPCR

Authors

  • Wafaa Abdul Kadhim Issa Department of Pathological Analysis, Faculty of Science, University of Kufa, Najaf, Iraq
  • Fadyia Mahdi Muslim Alameedy Department of Pathological Analysis, Faculty of Science, University of Kufa, Najaf, Iraq

Keywords:

renal failure, cytomegalovirus, qPCR

Abstract

Human samples from renal failure patients were collected from July 25, 2023, to September 19, 2023. The age range of the patients was 1 to 80 years. The RT-qPCR method was used to detect Cytomegalovirus in all samples; the results showed seventy-nine positive cases for Cytomegalovirus. The population groups studied were divided into five age groups: 1-16, 17-33, 34-50, 51-67, and 68-84 years. The fourth group (51-67) had the highest number of infected cases at 29.11%, compared to the age group 1-16 at 12.65%, 17-33 at 15.18%, 34-50 at 27.84%, and the age group 68-84 at 15.18%. The samples were collected from hospitals, including Al-Nasiriyah Teaching Hospital, Dialysis Center, and Al-Hussein Hospital.

References

Alkhaqani AL. Risk Factors and Complications of Chronic Kidney Disease: Narrative Review. Al-Rafidain Journal of Medical Sciences. 2022;2:107-114. https://doi.org/10.54133/ajms.v2i.68.

Al-Fayyadh ZA, Mezher MN. Immunological and Molecular Study of Human Cytomegalovirus contribution to Anemia in patients with Chronic Kidney Disease, 2020.

Berry R, Watson GM, Jonjic S, Degli-Esposti MA, Rossjohn J. Modulation of innate and adaptive immunity by cytomegaloviruses. Nat Rev Immunol. 2020;20(2):113-27.

Caplin B, Yang CW, Anand S, Levin A, Madero M, Saran R, et al. The International Society of Nephrology’s International Consortium of Collaborators on Chronic Kidney Disease of Unknown Etiology: Report of the working group on approaches to population-level detection strategies and recommendations for a minimum dataset. Kidney Int. 2019;95(1):4-10.

Feng JY, Zhang X. Post-transplant monitoring of lymphocyte counts predict the occurrence of CMV DNAemia in early phase of kidney transplantation, 2020.

Jackson SE, Redeker A, Arens R, Van Baarle D, Van Den Berg SPH, Benedict CA, et al. CMV immune evasion and manipulation of the immune system with aging. Geroscience. 2017;39(3):273-91.

Zuhair M, Smit GSA, Wallis G, et al. Estimation of the worldwide seroprevalence of cytomegalovirus: a systematic review and meta-analysis. Rev Med Virol. 2019;29(3):1-6.

Downloads

Published

2024-04-02

How to Cite

Issa, W. A. K., & Alameedy, F. M. M. (2024). Diagnosis of human cytomegalovirus with renal failure by RT-qPCR. Journal of Advance Multidisciplinary Research, 3(1), 64–66. Retrieved from https://synstojournals.com/multi/article/view/113

Issue

Section

Articles