A review on molecular characterization of feather degrading, phytostimulating and biocontrol potential
Keywords:
actinobacteria, feather degrading soil, 16S rRNA analysisAbstract
This observe turned into targeted at the isolation and characterization of Actinobacteria-generating keratinase enzyme from feather-dumping soil. A general of ten Actinobacterial cultures have been remoted on Actinomycetes Isolation Agar (AIA) which turned into specific as JJCPDKTA1 to JJCPDKTA10. The Actinobacterial isolates received have been subcultured on ISP 1 medium. The Primary screening of the isolates for keratinase enzyme interest turned into achieved on Skim Milk Agar. Based at the clearing of the medium, colonies have been subjected to secondary screening on a changed basal liquid medium which turned into supplemented with uncooked bird feathers. Out of ten handiest 3 isolates JJCPDKTA2, JJCPDKTA3 JJCPDKTA9 have been studied for morphological, physiological, and biochemical residences in addition to 16S rRNA series and phylogenetic analyses indicated that those Actinobacteria like Streptomyces variabilis, Streptomyces. Azureus and Actinomycetales. They have been deposited on the gene financial institution and get accession numbers i.e Streptomyces variabilis (KR909307), Streptomyces. Azureus (KR909308) and Actinomycetales (KR909309). The paintings concludes that actinobacteria to evaluate keratinase production. Finally, Actinobacteria with the pleasant capacity to degrade feathers by using keratinolytic interest.
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